 |
|
| | |
 PRODUCTS FOR BLOTTING, CHROMATOGRAPHY, AND HYBRIDIZATION
Click on the links below for information on each product:
|
|
|
|
| |
 |
Pure Nitrocellulose |
| |
 |
|
Use for blotting and hybridization:
|
Made of esters of nitrocellulose - free of acetate and other esters |
|
Binds up to 80-100μg/cm2 of proteins or nucleic acids |
|
Available in sheets, disks, or rolls |
| Contact us for more information on this line of products. |
|
 top of this page |
| |
|
Cellulose Acetate SELECA-V |
| |
|
|
Seleca-V cellulose acetate is specially made for electrophoresis:
| High purity membrane leaves almost no residue at application point; sharp separation with low varability |
| This cellulose acetate membrane is not for filtration. Click here for information on cellulose acetate membranes for filtration. |
| Produce consistent, reproducible results with minimal sample volume |
| Available in plain, white 60x220mm sheets |
| Use to confirm products by electrophoresis, in conjunction with TLC, PC, etc. |
| Contact us for more information on this line of products. |
|
|
Fractionate Density (g/dL)
|
Fractionate Density Ratio (%)
|
| Color |
white |
| Porosity |
72% |
| Refractive Index |
1.47 |
| Thickness |
180μm |
| Tensile Strength |
1.4kN/m |
| Electric Permeability |
-1mm* |
*migration distance of blue dextran
|
| Protein Fraction |
Average Value |
Common Range |
| Total Protein |
7.5 |
6.6~8.4 |
| Albumin |
4.92 |
4.21~5.63 |
| α1 |
0.20 |
0.14~0.26 |
| α2 |
0.46 |
0.30~0.62 |
| β |
0.76 |
0.54~0.98 |
| γ |
1.15 |
0.71~1.59 |
|
| Protein Fraction |
Average Value |
Common Range |
| Albumin |
65.8 |
58.9~72.6 |
| α1 |
2.6 |
1.8~3.4 |
| α2 |
6.2 |
4.2~8.2 |
| β |
10.1 |
7.7~12.5 |
| γ |
15.3 |
10.2~20.4 |
| A/G |
1.9 |
1.3~2.5 |
|
General Use
The following methods have been used for the preparation, electrophoresis, staining, etc., of this membrane.
|
| Sample Application |
Abot 1.2cm from the cathode side [(+8:2(-)] |
| Electricity Supplied |
0.4~0.8mA/cm |
| Electrophoresis Run Time |
25~40min |
| Staining |
Ponceau 3R dissolved in 6% trichloroacetic acid (for a 0.4~0.8% solution) |
| Destaining |
1~3% acetic acid |
| Developer |
Dekalin |
|
|
Chromatography Papers |
| |
|
|
Use for blotting, chromatography, and electrophoresis:
|
Several grades available, tested for spot formation, absorption speed, and flow rate for uniformity |
|
Click here for information on Paper Electrophoresis |
| Contact us for more information on this line of products. |
|
|
Grade |
Weight (g/m2) |
Thickness (mm) |
Ash Content (%) |
| 50 |
140 |
0.25 |
0.1 |
| 51A |
87 |
0.18 |
0.01 |
| 151B |
87 |
0.17 |
0.06 |
| 1514A |
185 |
0.32 |
0.06 |
| 526 |
325 |
0.70 |
0.1 |
|
| top of this page |
| |
|
Membranes
Pure Nitrocellulose